Differential expression of non-coding RNA in septic cardiomyocytes

doi:10.3877/cma.j.issn.1674-6880.2021.02.004

Abstract

Abstract:

Objective

To explore the differential expression levels of long non-coding RNA (lncRNA) and microRNA (miRNA) in septic cardiomyocytes.

Methods

The gene microarray was used to detect the differential lncRNA and miRNA in the cardiomyocytes of mice in the sepsis group and control group. The gene ontology and Kyoto Encyclopedia of Genes and Genomes were used to predict the major biological functions and signal pathways involved in the differentially expressed lncRNA and miRNA. Then the differential lncRNA and miRNA related to myocardial cell and mitochondrial injury were further screened through starbase and NCBI databases. The above related genes were verified and detected by real-time quantitative PCR.

Results

A total of 5 520 distinctly differentially expressed lncRNAs and 90 distinctly differentially expressed miRNAs were found in the sepsis group as compared with the control group, among which 3 186 lncRNAs and 59 miRNAs were up-regulated, and 2 334 lncRNAs and 31 miRNAs down-regulated. Biological function analysis showed that xist, 1700020I14Rik in lncRNA and miR-7a-5p, miR-30c-1-3p in miRNA were closely related to damage of cardiomyocytes and mitochondria. Compared with the control group, the expression levels of xist (t = 5.640, P = 0.002) and 1700020I14Rik (t = 6.044, P = 0.002) significantly increased, and the expression levels of miR-7a-5p (t = 27.277, P < 0.001) and miR-30c-1-3p (t = 12.910, P < 0.001) markedly decreased in the sepsis group.

Conclusion

Both lncRNA and miRNA are involved in the pathophysiological process of sepsis in cardiomyocytes, and the xist, 1700020I14Rik, miR-7a-5p and miR-30c-1-3p may play a key role in the sepsis-induced cardiac dysfunction.

Key words: Sepsis, Myocytes, cardiac, Non-coding RNA

Airong Huang, Dongshi Liang, Yimei Jin, Miaomiao Lin, Xiaojiao Xia, Xiaoli Chen. Differential expression of non-coding RNA in septic cardiomyocytes[J]. Chinese Journal of Critical Care Medicine(Electronic Edition), 2021, 14(02): 113-119.

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URL: https://zhwzzyxzz.cma-cmc.com.cn/EN/10.3877/cma.j.issn.1674-6880.2021.02.004

https://zhwzzyxzz.cma-cmc.com.cn/EN/Y2021/V14/I02/113

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References 34

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引物名称	序列
miR-7a-5p上游引物	GCCGAGGGAAGACTAGTGAT
miR-7a-5p下游引物	CTCAACTGGTGTCGTGGAGT
miR-30c-1-3p上游引物	CGAGTGGGAGAGGGTTGT
miR-30c-1-3p下游引物	CTCAACTGGTGTCGTGGAGT
U6上游引物	CTCGCTTCGGCAGCACA
U6下游引物	AACGCTTCACGAATTTGCGT
1700020I14Rik上游引物	TCCCGTGAGAAGCGGAGTA
1700020I14Rik下游引物	CGTGCCAAGTCCTGAGTTCA
xist上游引物	GGCTCAAAGAATGCCACCCT
xist下游引物	GATCACGCTGAAGACCCAGTT
GAPDH上游引物	AGGTCGGTGTGAACGGATTTG
GAPDH下游引物	TGTAGACCATGTAGTTGAGGTCA

引物名称	序列
miR-7a-5p上游引物	GCCGAGGGAAGACTAGTGAT
miR-7a-5p下游引物	CTCAACTGGTGTCGTGGAGT
miR-30c-1-3p上游引物	CGAGTGGGAGAGGGTTGT
miR-30c-1-3p下游引物	CTCAACTGGTGTCGTGGAGT
U6上游引物	CTCGCTTCGGCAGCACA
U6下游引物	AACGCTTCACGAATTTGCGT
1700020I14Rik上游引物	TCCCGTGAGAAGCGGAGTA
1700020I14Rik下游引物	CGTGCCAAGTCCTGAGTTCA
xist上游引物	GGCTCAAAGAATGCCACCCT
xist下游引物	GATCACGCTGAAGACCCAGTT
GAPDH上游引物	AGGTCGGTGTGAACGGATTTG
GAPDH下游引物	TGTAGACCATGTAGTTGAGGTCA

组别	浓度（ng/μL）	A₂₆₀/A₂₈₀	A₂₆₀/A₂₃₀
对照组	777.7 ± 1.6	1.89 ± 0.07	1.931 ± 0.019
脓毒症组	865.3 ± 3.7	1.85 ± 0.05	1.975 ± 0.043

组别	浓度（ng/μL）	A₂₆₀/A₂₈₀	A₂₆₀/A₂₃₀
对照组	777.7 ± 1.6	1.89 ± 0.07	1.931 ± 0.019
脓毒症组	865.3 ± 3.7	1.85 ± 0.05	1.975 ± 0.043

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