Effect of recombinant human thrombopoietin on proliferation in human hepatic cell line L02

doi:10.3877/cma.j.issn.1674-6880.2020.03.002

Abstract

Abstract:

Objective

To investigate the effect of recombinant human thrombopoietin (rhTPO) on healthy human hepatic cell line L02.

Methods

The survival rate of L02 cells was observed among a control group (0 U rhTPO), a low-dose group (150 U rhTPO) and a high-dose group (750 U rhTPO) according to different doses of rhTPO. The expression levels of extracellular signal regulated kinase (ERK) and phosphorylation-ERK (p-ERK) protein were detected by Western-blotting, and the expression level of ERK messenger RNA (mRNA) was examined by fluorogenic quantitative PCR in the L02 cells among group A (treated with 0 U rhTPO), group B (treated with 15 U rhTPO), group C (treated with 150 U rhTPO) and group D (treated with 1 500 U rhTPO).

Results

The survival rate of L02 cells was significantly different among the three groups (F = 8.843, P = 0.016). It was much higher in the low-dose group and high-dose group than in the control group, and highest in the high-dose group [(107.9 ± 4.0)%, (112.0 ± 1.6)%, (100.0 ± 2.5)%; all P < 0.05]. However, there were no statistically significant differences in the expression levels of ERK protein and ERK mRNA among the four groups (F = 1.140, P = 0.392; F = 0.437, P = 0.733). Besides, the expression level of p-ERK protein was significantly different among the four groups (F = 31.400, P < 0.001). It increased obviously in group B, group C and group D as compared with group A (all P < 0.05), while no significant differences were noted among group B, group C and group D (all P > 0.05).

Conclusion

rhTPO can promote the proliferation of L02 hepatocytes by activating ERK.

Key words: Human hepatic cell line, Recombinant human thrombopoietin, Cell proliferation

Danli Yu, Ling Cai, Dongsheng Qi, Shusen Zheng, Weifang Zhang. Effect of recombinant human thrombopoietin on proliferation in human hepatic cell line L02[J]. Chinese Journal of Critical Care Medicine(Electronic Edition), 2020, 13(03): 165-169.

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Figures/Tables 3

References 20

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组别	细胞数（个/ mL）	存活率
对照组	1 × 10⁴	100.0 ± 2.5
低剂量组	1 × 10⁴	107.9 ± 4.0^a
高剂量组	1 × 10⁴	112.0 ± 1.6^ab
F值	?	8.843
P值	?	0.016

组别	细胞数（个/ mL）	存活率
对照组	1 × 10⁴	100.0 ± 2.5
低剂量组	1 × 10⁴	107.9 ± 4.0^a
高剂量组	1 × 10⁴	112.0 ± 1.6^ab
F值	?	8.843
P值	?	0.016

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