Intervention effect of invigorating kidney and activating blood in mice with chronic aplastic anemia

doi:10.3877/cma.j.issn.1674-6880.2018.02.002

Abstract

Abstract:

Objective

To explore the intervention effect of invigorating kidney and activating blood in mice with chronic aplastic anemia (CAA) and its mechanism.

Methods

The mice model of kidney deficiency complicated with CAA was established. Totally 30 mice were divided into the medicine group, model group and control group, 10 mice in each group. Mice in the medicine group were given orally herbal soup (0.2 mL each time, 2 times a day for 60 days), and mice in the model group and control group only received the same dose of normal saline. The general condition was observed. The levels of white blood cells, hemoglobin and platelets were detected after injection of benzene for 16 and 25 times. The levels of serum vascular endothelial growth factor (VEGF) and stromal cell-derived factor-1a (SDF-1a) were determined by enzyme linked immunosorbant assay. Meanwhile, the bone marrow nucleated cells were counted under electron microscope, and the bone marrow pathological features were observed through hatmatoxylin-eosin (HE) staining.

Results

The mice in the medicine group and model group gradually showed weight loss, fatigue, dry and sparse hair, pale limbs and so on, and as the Chinese medicine intervention prolonged, the mice in the medicine group recovered to varying degrees. The levels of white blood cells, hemoglobin and platelets all showed significant differences among three groups (F=16.536, 9.273, 5.667; all P<0.05). Above indicators at the same time in the model group and medicine group were much lower than those in the control group (all P<0.05), and above indicators after benzene injection for 25 times in the medicine group were much higher than those in the model group (all P<0.05). The levels of VEGF, SDF-1a and bone marrow nucleated cells also showed significant differences among three groups (F=11.231, 7.924, 8.455; all P<0.05). The levels of VEGF [(342 ± 11), (235 ± 13), (278 ± 13) ng/L], SDF-1a [(110 ± 13), (85 ± 11), (93 ± 12) ng/L] and bone marrow nucleated cells [(94 ± 15), (72 ± 11), (83 ± 13)/HP] in the model group and medicine group decreased more obviously compared with the control group, and they decreased most in the model group (all P<0.05). HE staining revealed that granulocyte, erythrocyte and megakaryocyte series decreased in the model group and medicine group, and the model group was less severe.

Conclusion

Invigorating kidney and activating blood may improve mice with CAA through enhancing the levels of serum VEGF and SDF-1a.

Key words: Anemia, aplastic, Mice, Intervention studies, Invigorating kidney and activating blood

Ju Sun, Mei Liu, Dijiong Wu, Wenbin Liu, Huijin Hu, Baodong Ye. Intervention effect of invigorating kidney and activating blood in mice with chronic aplastic anemia[J]. Chinese Journal of Critical Care Medicine(Electronic Edition), 2018, 11(02): 78-82.

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References 21

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组别	次数	例数	白细胞（× 10⁹/L）	血红蛋白（g/L）	血小板（× 10⁹/L）
对照组	16	10	11.2 ± 1.2	171 ± 13	1 119 ± 125
?	25	10	9.8 ± 1.1	165 ± 13	987 ± 146
模型组	16	8	4.6 ± 1.2^a	125 ± 12^ab	890 ± 113^a
?	25	8	2.8 ± 1.1^ab	111 ± 11^ab	719 ± 93^ab
中药组	16	9	5.1 ± 0.9^a	133 ± 15^a	812 ± 114^a
?	25	9	4.8 ± 1.3^a	122 ± 13^a	814 ± 111^a
F值	?	?	16.536	9.273	5.667
P值	?	?	<0.001	0.009	0.035

组别	次数	例数	白细胞（× 10⁹/L）	血红蛋白（g/L）	血小板（× 10⁹/L）
对照组	16	10	11.2 ± 1.2	171 ± 13	1 119 ± 125
?	25	10	9.8 ± 1.1	165 ± 13	987 ± 146
模型组	16	8	4.6 ± 1.2^a	125 ± 12^ab	890 ± 113^a
?	25	8	2.8 ± 1.1^ab	111 ± 11^ab	719 ± 93^ab
中药组	16	9	5.1 ± 0.9^a	133 ± 15^a	812 ± 114^a
?	25	9	4.8 ± 1.3^a	122 ± 13^a	814 ± 111^a
F值	?	?	16.536	9.273	5.667
P值	?	?	<0.001	0.009	0.035

组别	例数	VEGF	SDF-1a
对照组	10	342 ± 11	110 ± 13
模型组	8	235 ± 13^ab	85 ± 11^ab
中药组	9	278 ± 13^a	93 ± 12^a
F值	?	11.231	7.924
P值	?	0.006	0.014

组别	例数	VEGF	SDF-1a
对照组	10	342 ± 11	110 ± 13
模型组	8	235 ± 13^ab	85 ± 11^ab
中药组	9	278 ± 13^a	93 ± 12^a
F值	?	11.231	7.924
P值	?	0.006	0.014

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