Expression of miRNA-21 and transforming growth factor beta 1 in the lung fibrosis tissue during acute respiratory distress syndrome in rats

doi:10.3877/cma.j.issn.1674-6880.2016.04.005

Abstract

Abstract:

Objective

To observe the expression of miRNA-21 (miR-21) and transforming growth factor beta 1 (TGF-β1) in the lung fibrosis tissue during acute respiratory distress syndrome (ARDS) in rats.

Methods

A total of 72 rats were randomly divided into the control group and experimental group, 36 rats in each group. Rats in the control group received laparotomy, and the cecum was just taken out and returned; rats in the experimental group were reproduced by cecal ligation and puncture method. Six rats in each group were sacrificed at 12 h, 1 d, 2 d, 3 d, 4 d, and 5 d after operation. The left lung tissue was collected and observed by a microscope. The expression of miR-21 in lung tissues were measured through fluorescence real-time quantitative PCR. The expression of TGF-β1 were examined by immunohistochemistry staining.

Results

Compared with the control group, rats in the experimental group acted out lower activities, and slow in reacting and polypnea. Under the microscope, rats in the experimental group were observed a large of infiltration on inflammatory cells and red cells, fibroblasts and hyperplasia of fibrotic tissue, but rats in the control group showed no inflammatory cell infiltration and collagen deposition in the lung. The expression of the miR-21 from 2-5 d after operation in the experimental group were higher than those in the control group at the same time point (t=7.121, 5.330, 6.513, 19.371, all P<0.05). The positive expression rate of TGF-β1 in the experimental group were 22.64%, 32.69%, 36.20%, 38.32%, and 45.64%, respectively on 1-5 d after operation, and were 0 in the control group.

Conclusions

The expression of the miR-21 and TGF-β1 were increased in the lung fibrosis tissues induced by ARDS. miR-21 and TGF-β1 play important roles in the ARDS rats with pulmonary fibrosis.

Key words: MicroRNAs, Transforming growth factor beta 1, Acute respiratory distress syndrome, Pulmonary fibrosis

Junyi Chen, Yichun Wang. Expression of miRNA-21 and transforming growth factor beta 1 in the lung fibrosis tissue during acute respiratory distress syndrome in rats[J]. Chinese Journal of Critical Care Medicine(Electronic Edition), 2016, 09(04): 234-239.

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