Effects of prone ventilation in rats with pulmonary acute lung injury

doi:10.3877/cma.j.issn.1674-6880.2016.01.002

Abstract

Abstract:

Objective

To investigate the effects of prone ventilation in rats with lipopolysaccharide (LPS) induced pulmonary acute lung injury (ALI).

Methods

Totally 32 male Sprague-Dawley rats were randomly divided into four groups, 8 rats in each group: control group, pulmonary ALI group, supine position group (ALIS) and prone position group (ALIP). Rats in the ALI, ALIS, and ALIP groups were given 6 mg/kg LPS intratracheally to build the ALI rat model. After 24 h, rats in the ALIS and ALIP groups were were mechanically ventilated for 4 h in supine or prone positions. After that, PaO₂ and PaCO₂ of arterial blood, lung tissue wet/dry weight ratio (W/D), tumour necrosis factor-alpha (TNF-α) and interleukin-6(IL-6) concentrations in serum were compared in these four groups, and lung tissue morphometric analysis was inverstigated under light microscope at the same time.

Results

Compared with the control group, PaO₂ were significantly lower in the ALI, ALIS and ALIP groups (F = 57.369, P < 0.001); PaCO₂ in the ALI and ALIS groups were obviously higher (F = 8.448, P < 0.001); lung tissue W/D ratio, as well as the levels of TNF-α and IL-6 in serum were significantly higher in the ALI, ALIS and ALIP groups (F = 13.609, 6.443, 23.849; P = 0.001, 0.017, < 0.001). Compared with the ALI group, PaO₂ in the ALIS and ALIP groups was significantly higher, but lung tissue W/D ratio, TNF-α and IL-6 in serum were obviously lower in these two groups (all P< 0.05). Compared with the ALIS group, except PaO₂ was higher [(83 ± 6) mmHg vs. (71 ± 5) mmHg], PaCO₂ [(50 ± 7) mmHg vs. (58 ± 6) mmHg, P < 0.05], lung tissue W/D ratio [(4.65 ± 0.56) vs. (4.82 ± 0.41), P < 0.05, TNF-α and IL-6 in serum [(293 ± 68) ng/L vs. (366 ± 44) ng/L; (358 ± 39) ng/L vs. (440 ± 38) ng/L; all P < 0.05] were obviously lower in the ALIP group. Meanwhile under light microscope, compared with the ALIS group, hyper-inflated areas were less (F = 63.423, P < 0.001), normal areas were larger (F = 73.229, P < 0.001) on the ventral side in the ALIP group. Also, normal and hyper-inflated area were greater (F = 61.871, P < 0.001; F = 9.800; P = 0.001) when the collapsed area was less (F = 68.586, P < 0.001) on the dorsal side in the ALIP group.

Conclusion

In LPS-induced pulmonary ALI rats model, the prone ventilation improved gas exchange, decreased pulmonary edema with attenuate inflammatory responses.

Key words: Prone ventilation, Acute lung injury, Lipopolysaccharides, Rat, Sprague-Dawley

Maoxian Yang, Yunchao Shi, Jiangang Zhu, Peng Shen, Haibo Wang, Xianbin Song, Pengcheng Xu, Wenjing Zhao. Effects of prone ventilation in rats with pulmonary acute lung injury[J]. Chinese Journal of Critical Care Medicine(Electronic Edition), 2016, 09(01): 9-13.

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References 20

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组别	例数	PaO₂(mmHg)	PaCO₂(mmHg)	肺组织W/D	血清TNF-α(ng/L)	血清IL-6(ng/L)
对照组	8	115 ± 9	43 ± 6	4.43 ± 0.18	206 ± 18	116 ± 27
ALI组	8	57 ± 4^a	58 ± 6^a	5.06 ± 0.49^a	443 ± 49^a	645 ± 78^a
ALIS组	8	71 ± 5^ab	50 ± 7^ab	4.82 ± 0.41^ab	366 ± 44^ab	440 ± 38^ab
ALIP组	8	83 ± 6^abc	42 ± 6^bc	4.65 ± 0.56^abc	293 ± 68^abc	358 ± 39^abc
F值	?	57.369	8.448	13.609	6.443	23.849
P值	?	< 0.001	< 0.001	0.001	0.017	< 0.001

组别	例数	PaO₂(mmHg)	PaCO₂(mmHg)	肺组织W/D	血清TNF-α(ng/L)	血清IL-6(ng/L)
对照组	8	115 ± 9	43 ± 6	4.43 ± 0.18	206 ± 18	116 ± 27
ALI组	8	57 ± 4^a	58 ± 6^a	5.06 ± 0.49^a	443 ± 49^a	645 ± 78^a
ALIS组	8	71 ± 5^ab	50 ± 7^ab	4.82 ± 0.41^ab	366 ± 44^ab	440 ± 38^ab
ALIP组	8	83 ± 6^abc	42 ± 6^bc	4.65 ± 0.56^abc	293 ± 68^abc	358 ± 39^abc
F值	?	57.369	8.448	13.609	6.443	23.849
P值	?	< 0.001	< 0.001	0.001	0.017	< 0.001

组别	例数	背侧区域			腹侧区域
组别	例数	正常肺泡(%)	塌陷肺泡(%)	过度膨胀肺泡(%)	正常肺泡(%)	塌陷肺泡(%)	过度膨胀肺泡(%)
对照组	8	96.6 ± 2.3	3.4 ± 2.3	0.0 ± 0.0	94.1 ± 2.0	5.9 ± 2.0	0.0 ± 0.0
ALI组	8	64.6 ± 4.7^a	35.4 ± 4.7^a	0.0 ± 0.0	60.3 ± 5.6^a	39.7 ± 5.6^a	0.0 ± 0.0
ALIS组	8	59.2 ± 4.8^ab	39.9 ± 4.9^ab	0.9 ± 0.6^ab	65.9 ± 7.0^ab	21.9 ± 5.9^ab	12.2 ± 3.4^ab
ALIP组	8	72.9 ± 4.7^abc	25.5 ± 5.0^abc	1.6 ± 1.1^abc	74.6 ± 3.5^bc	25.4 ± 3.5^ab	0.0 ± 0.0^c
F值	?	61.871	68.586	9.800	73.229	23.315	63.423
P值	?	< 0.001	< 0.001	0.001	< 0.001	< 0.001	< 0.001

组别	例数	背侧区域			腹侧区域
组别	例数	正常肺泡(%)	塌陷肺泡(%)	过度膨胀肺泡(%)	正常肺泡(%)	塌陷肺泡(%)	过度膨胀肺泡(%)
对照组	8	96.6 ± 2.3	3.4 ± 2.3	0.0 ± 0.0	94.1 ± 2.0	5.9 ± 2.0	0.0 ± 0.0
ALI组	8	64.6 ± 4.7^a	35.4 ± 4.7^a	0.0 ± 0.0	60.3 ± 5.6^a	39.7 ± 5.6^a	0.0 ± 0.0
ALIS组	8	59.2 ± 4.8^ab	39.9 ± 4.9^ab	0.9 ± 0.6^ab	65.9 ± 7.0^ab	21.9 ± 5.9^ab	12.2 ± 3.4^ab
ALIP组	8	72.9 ± 4.7^abc	25.5 ± 5.0^abc	1.6 ± 1.1^abc	74.6 ± 3.5^bc	25.4 ± 3.5^ab	0.0 ± 0.0^c
F值	?	61.871	68.586	9.800	73.229	23.315	63.423
P值	?	< 0.001	< 0.001	0.001	< 0.001	< 0.001	< 0.001

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