切换至 "中华医学电子期刊资源库"
高级检索
中华危重症医学杂志(电子版)

中华危重症医学杂志(电子版) ›› 2017, Vol. 10 ›› Issue (05) : 316 -321. doi: 10.3877/cma.j.issn.1674-6880.2017.05.005

所属专题： 文献；

论著

心肺复苏后大鼠血浆中微小核糖核酸在心肌损伤中的变化及其意义
江哲龙1, 王东升2, 何爱文2, 许华清2, 周志良2, 陈寿权2, 李章平2,†(), 李惠萍2, 黄唯佳2, 程俊彦2   
  1. 1. 310006　杭州，杭州市第一人民医院急诊科
    2. 325000　浙江温州，温州医科大学附属第一医院急诊科
  • 收稿日期:2016-08-16 出版日期:2017-10-01
  • 通信作者: 李章平
  • 基金资助:
    浙江省自然科学基金项目(LY16 h150008); 温州市科技项目(Y20140675、Y20090241)

Changes of plasma microRNA of rats in myocardial injury after cardiopulmonary resuscitation and their significance

Zhelong Jiang1, Dongsheng Wang2, Aiwen He2, Huaqing Xu2, Zhiliang Zhou2, Shouquan Chen2, Zhangping Li2,†(), Huiping Li2, Weijia Huang2, Junyan Cheng2   

  1. 1. Department of Emergency, Hangzhou First People's Hospital, Hangzhou 310006, China
    2. Department of Emergency, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China
  • Received:2016-08-16 Published:2017-10-01
  • Corresponding author: Zhangping Li
  • About author:
    Corresponding author: Li Zhangping, Email:
全文 ( ) 下载PDF ( ) 导出引用
引用本文：

江哲龙, 王东升, 何爱文, 许华清, 周志良, 陈寿权, 李章平, 李惠萍, 黄唯佳, 程俊彦. 心肺复苏后大鼠血浆中微小核糖核酸在心肌损伤中的变化及其意义[J/OL]. 中华危重症医学杂志(电子版), 2017, 10(05): 316-321.

Zhelong Jiang, Dongsheng Wang, Aiwen He, Huaqing Xu, Zhiliang Zhou, Shouquan Chen, Zhangping Li, Huiping Li, Weijia Huang, Junyan Cheng. Changes of plasma microRNA of rats in myocardial injury after cardiopulmonary resuscitation and their significance[J/OL]. Chinese Journal of Critical Care Medicine(Electronic Edition), 2017, 10(05): 316-321.

目的

本研究探讨心肺复苏后大鼠血浆中心肌损伤相关微小核糖核酸（miRNA）的变化及其意义。

方法

将64只Sprague-Dawley大鼠分为对照组和实验组，每组各32只。采用窒息法建立大鼠心肺复苏模型，两组分别于气管切开后（对照组）或心肺复苏后（实验组）3、6、12、24 h取静脉血，检测各时间点血浆miRNA-1、miRNA-21、miRNA-126、miRNA-133a、miRNA-210、miRNA-320及miRNA-499的表达水平，并采用受试者工作特征（ROC）曲线的曲线下面积评价两组大鼠血浆各miRNA对心搏骤停-心肺复苏后心肌损伤的预测价值。

结果

实验组与对照组间不同时间点miRNA-1、miRNA-21、miRNA-126、miRNA-133a、miRNA-210、miRNA-320和miRNA-499相对表达量比较，差异均有统计学意义（F = 223.037、635.809、51.557、167.260、93.157、296.361、622.023，P均< 0.001）；进一步两两比较发现，miRNA-1、miRNA-133a和miRNA-499在各时间点的相对表达量较对照组均明显上升（P均< 0.05）；而miRNA-21在各时间点相对表达量较对照组均显著下降（P均< 0.001）；此外，miRNA-126在3、6、12 h的相对表达量较对照组均明显下调（P均< 0.001），而在24 h的相对表达量与对照组比较则显著上调（P < 0.001）。miRNA-210在6 h、12 h的相对表达量和miRNA-320在3、6、12 h的相对表达量较对照组均呈上调趋势（P均< 0.05）。实验组中，miRNA-1，miRNA-133a，miRNA-210在3 h的相对表达量与24 h的相对表达量比较，差异均无统计学意义（P均> 0.05），其余各时点比较差异均具有统计学意义（P均< 0.05）；miRNA-21和miRNA-126在3 h与6 h的相对表达量比较、6 h与12 h的相对表达量比较，差异均无统计学意义（P均> 0.05），其余各时间点间的相对表达量比较，差异均有统计学意义（P均< 0.05）；而miRNA-320和miRNA-499各个时间点的相对表达量比较，差异均有统计学意义（P均< 0.05）。ROC曲线分析结果显示miRNA-1、miRNA-21、miRNA-126、miRNA-133a、miRNA-320及miRNA-499的ROC曲线下面积分别为：0.938、0.912、0.859、0.914、0.805和0.969，敏感度分别为：0.750、1.000、0.875、0.750、1.000和0.875，特异度分别为：1.000、0.750、0.750、1.000、0.750和1.000（P均< 0.05），提示miRNA-1、miRNA-21、miRNA-126、miRNA-133a、miRNA-320及miRNA-499对心肺复苏后心肌损伤具有预测价值。

结论

大鼠心肺复苏后血浆中各miRNA的相对表达量在心肌损伤中有不同的变化，其中miRNA-1、miRNA-21、miRNA-126、miRNA-133a、miRNA-320及miRNA-499对心肺复苏后心肌损伤均具有预测价值。

关键词: 心肺复苏, 心肌损伤, 微小核糖核酸, 大鼠
Objective

To study changes of plasma microRNA (miRNA) of rats in myocardial injury after cardiopulmonary resuscitation and their significance.

Methods

A total of 64 Sprague-Dawley rats were divided into the control group and the experimental group, 32 rats in each group. The rat model of cardiopulmonary resuscitation was established by asphyxia. The levels of plasma miRNA-1, miRNA-21, miRNA-126, miRNA-133a, miRNA-210, miRNA-320 and miRNA-499 were detected at different time points when the two groups received venous blood after tracheotomy (the control group) or cardiopulmonary resuscitation (the experimental group) 3, 6, 12, 24 h respectively. The area under the receiver operating characteristic (ROC) curve was used to evaluate the predictive value of plasma miRNA for myocardial injury after cardiac arrest-cardiopulmonary resuscitation in the two groups.

Results

The relative values of miRNA-1, miRNA-21, miRNA-126, miRNA-133a, miRNA-210, miRNA-320 and miRNA-499 in the experimental group showed statistical differences as compared with the control group in different time pionts (F = 223.037, 635.809, 51.557, 167.260, 93.157, 296.361, 622.023, all P < 0.001). In comparison with the control group, the relative expressions of miRNA-1, miRNA-133a and miRNA-499 in the experimental group significantly increased in each time point (all P < 0.05), while the relative expression of miRNA-21 all reduced (all P < 0.001). In addition, the relative expression of miRNA-126 in the experimental group at 3, 6 and 12 h were significantly lower than that in the control group (all P < 0.001); however it at 24 h was significantly higher than that in the control group (P < 0.001). The relative expressions of miRNA-210 at 6 h and 12 h and miRNA-320 at 3, 6 and 12 h in the experimental group showed an upward trend as compared with the control group (all P < 0.05). In the experimental group, the relative expressions of miRNA-1, miRNA-133a and miRNA-210 at 3 h showed no significant differences as compared with those at 24 h (all P > 0.05), while there were significant differences in the rest of time points (all P < 0.05). At the same time, the relative expressions of miRNA-21 and miRNA-126 between 3 h and 6 h, 6 h and 12 h had no statistically significant differences (all P > 0.05), but there were significant differences in the rest of time points (all P < 0.05). However, the relative expressions of miRNA-320 and miRNA-499 at each time point had statistical differences (all P < 0.05). ROC curve analysis results showed that the areas under the ROC curve of miRNA-1, miRNA-21, miRNA-126, miRNA-133a, miRNA-320 and miRNA-499 were 0.938, 0.912, 0.859, 0.914, 0.805 and 0.969 respectively, their sensitivity were 0.750, 1.000, 0.875, 0.750, 1.000 and 0.875, and their specificity were 1.000, 0.750, 0.750, 1.000, 0.750 and 1.000 (all P < 0.05), which suggested that miRNA-1, miRNA-21, miRNA-126, miRNA-133a, miRNA-320 and miRNA-499 had predictive value for myocardial injury after cardiopulmonary resuscitation.

Conclusion

The relative expression of plasma miRNA of rats has different changes in myocardial injury after cardiopulmonary resuscitation, and miRNA-1, miRNA-21, miRNA-126, miRNA-133a, miRNA-320 and miRNA-499 have predictive value for myocardial injury after cardiopulmonary resuscitation.

Key words: Cardiopulmonary resuscitation, Myocardial injury, MicroRNA, Rat
表1 两组大鼠基本资料比较（ ± s）
组别 只数 体质量（g） 水合氯醛用量（mL） MAP基础值（mmHg）
实验组 32 300 ± 17 2.10 ± 0.12 104 ± 8
对照组 32 298 ± 17 2.07 ± 0.11 105 ± 7
t值 ? 0.412 1.095 0.721
P值 ? 0.657 0.237 0.475
图1 实验组与对照组大鼠不同时间点血浆miRNA-1、miRNA-21、miRNA-126、miRNA-133a、miRNA-210、miRNA-320和miRNA-499相对表达量的比较。注：与对照组比较，*P < 0.05；与实验组3 h比较，#P < 0.05；与实验组6 h比较，+P < 0.05；与实验组12 h比较，&P < 0.05
1
何爱文，陈寿权. 心肺复苏研究的最新进展[J/CD]. 中华危重症医学杂志（电子版），2012，5(4):218-223.
2
孙晓莉，郑雪冰，王蕊，等. 生脉注射液对心肺复苏中心肌再灌注损伤的保护作用[J]. 中国实验诊断学, 2015，19(8):1334-1336.
3
Fasanaro P, D'Alessandra Y, Magenta A, et al. Micro-RNAs: promising biomarkers and therapeutic targets of acute myocardial ischemia[J]. Curr Vasc Pharmacol, 2015, 13 (3): 305-315.
4
Mitchell PS, Parkin RK, Kroh EM, et al. Circulating microRNAs as stable blood-based markers for cancer detection[J]. Proc Natl Acad Sci USA, 2008, 105 (30): 10513-10518.
5
任妙丹，何爱文，陈寿权，等. 心肺复苏后大鼠皮质区凋亡相关性微小RNA的表达变化[J]. 中国中西医结合急救杂志，2014，21(2):95-98.
6
Boller M, Fletcher DJ, Brainard BM, et al. Utsteinstyle guidelines on uniform reporting of in-hospital cardiopulmonary resuscitation in dogs and cats[J]. J Vet Emerg Crit Care (San Antonio), 2016, 26 (1): 11-34.
7
杨祖军，徐文卫，汪群智，等. 参附注射液对心肺复苏后心功能不全患者血流动力学的影响[J/CD]. 中华危重症医学杂志（电子版），2014，7(4):266-268.
8
Song R, Ro S, Michaels JD, et al. Many X-linked microRNAs escape meiotic sex chromosome inactivation[J]. Nat Genet, 2009, 41 (4): 488-493.
9
Huan L, Liang LH, He XH. Role of microRNAs in inflammation-associated liver cancer[J]. Cancer Biol Med, 2016, 13 (4): 407-425.
10
Stroun M, Anker P, Beljanski M, et al. Presence of RNA in the nucleoprotein complex spontaneously released by human lymphocytes and frog auricles in culture[J]. Cancer Res, 1978, 38 (10): 3546-3554.
11
Klug G, Metzler B. The role of circulating microRNAs in acute coronary syndromes: ready for prime time?[J]. Ann Transl Med, 2016, 4 (24): 537.
12
Mishra PK, Tyagi N, Kumar M, et al. MicroRNAs as a therapeutic target for cardiovascular diseases[J]. J Cell Mol Med, 2009, 13 (4): 778-789.
13
Duan L, Xiong X, Liu Y, et al. MiRNA-1: functional roles and dysregulation in heart disease[J]. Mol Biosyst, 2014, 10 (11): 2775-2782.
14
Shan ZX, Lin QX, Deng CY, et al. miRNA-1/miRNA206 regulate Hsp60 expression contributing to glucose-mediated apoptosis in cardiomyocytes[J]. FEBS Lett, 2010, 584 (16): 3592-3600.
15
Li S, Xiao FY, Shan PR, et al. Overexpression of microRNA-133a inhibits ischemia-reperfusion-induced cardiomyocyte apoptosis by targeting DAPK2[J]. J Hum Genet, 2015, 60 (11): 709-716.
16
Wang GK, Zhu JQ, Zhang JT, et al. Circulating microRNA: a novel potential biomarker for early diagnosis of acute myocardial infarction in humans[J]. Eur Heart J, 2010, 31 (6): 659-666.
17
Dong S, Cheng Y, Yang J, et al. MicroRNA expression signature and the role of microRNA-21 in the early phase of acute myocardial infarction[J]. J Biol Chem, 2009, 284 (43): 29514-29525.
18
He F, Ren Y, Shi E, et al. Overexpression of micro-RNA-21 protects spinal cords against transient ischemia [J]. J Thorac Cardiovasc Surg, 2016, 152 (6): 1602-1608.
19
Tagscherer KE, Fassl A, Sinkovic T, et al. MicroRNA-210 induces apoptosis in colorectal cancer via induction of reactive oxygen[J]. Cancer Cell Int, 2016 (16): 42.
20
Ren XP, Wu J, Wang X, et al. MicroRNA-320 is involved in the regulation of cardiac ischemia/reperfusion injury by targeting heart-shock protein 20[J]. Circulation, 2009, 119 (17): 2357-2366.
21
Mondadori dos Santos A, Metzinger L, Haddad O, et al. miRNA-126 is involved in vascular remodeling under laminar shear stress[J]. Biomed Res Int, 2015, 497280.
22
Togliatto G, Trombetta A, Dentelli P, et al. Unacylated ghrelin induces oxidative stress resistance in a glucose intolerance and peripheral artery disease mouse model by restoring endothelial cell miRNA-126 expression[J]. Diabetes, 2015, 64 (4): 1370-1382.
23
Wang JN, Yan YY, Guo ZY, et al. Negative association of circulating microRNA-126 with high-sensitive C-reactive rrotein and vascular cell adhesion molecule-1 in patients with coronary artery disease following percutaneous coronary intervention[J]. Chin Med J (Engl), 2016, 129 (23): 2786-2791.
24
Zhu J, Yao K, Wang Q, et al. Ischemic postconditioning-regulated miRNA-499 protects the rat heart against ischemia/reperfusion injury by inhibiting apoptosis through PDCD4[J]. Cell Physiol Biochem, 2016, 39 (6): 2364-2380.
25
Zhang L, Chen X, Su T, et al. Circulating miRNA-499 are novel and sensitive biomarker of acute myocardial infarction[J]. J Thorac Dis, 2015, 7 (3): 303-308.
[1] 刘思锐, 赵辰阳, 张睿, 张一休, 杨萌. 多普勒超声对孕鼠子宫动脉不同节段血流动力学参数的评估[J/OL]. 中华医学超声杂志（电子版）, 2024, 21(09): 877-883.
[2] 李朋, 苗立帅, 朱智奇. 四氢嘧啶对大鼠关节软骨细胞的保护作用[J/OL]. 中华关节外科杂志(电子版), 2024, 18(01): 69-77.
[3] 林琳, 田思萌, 于永华, 徐飞飞, 黄明莉. 干细胞及其外泌体治疗宫腔黏连的研究现状[J/OL]. 中华妇幼临床医学杂志(电子版), 2024, 20(03): 271-275.
[4] 王晓亚, 王燕芸, 顾永忠. 产科快速反应团队成功救治心脏骤停孕产妇1例并文献复习[J/OL]. 中华妇幼临床医学杂志(电子版), 2024, 20(02): 148-156.
[5] 邓健, 王少华, 陈尊, 邹振庄. Keap1/Nrf2信号通路在脂多糖诱导宫内感染致新生鼠支气管肺发育不良的作用机制[J/OL]. 中华妇幼临床医学杂志(电子版), 2023, 19(06): 665-674.
[6] 杨文飞, 郝嘉文, 鲁梦远, 赵学刚, 李聪颖, 盖晨阳, 张晶, 张庆富. 高压电烧伤对大鼠心肌氧化应激的影响及N-乙酰半胱氨酸的干预作用[J/OL]. 中华损伤与修复杂志(电子版), 2024, 19(02): 106-112.
[7] 李晓玉, 江庆, 汤海琴, 罗静枝. 围手术期综合管理对胆总管结石并急性胆管炎患者ERCP +LC术后心肌损伤的影响研究[J/OL]. 中华普外科手术学杂志(电子版), 2024, 18(01): 57-60.
[8] 刘中文, 刘畅, 高洋, 刘东, 林世庆, 杨建华, 赵福义. 尿液microRNA-326与腹腔镜根治性膀胱切除术治疗膀胱癌患者预后的相关性研究[J/OL]. 中华腔镜泌尿外科杂志(电子版), 2024, 18(04): 386-391.
[9] 张礼刚, 邹志辉, 许顺, 蔡可可, 胡永涛, 梁朝朝. 酒精对慢性非细菌性前列腺炎中T淋巴细胞变化的影响研究[J/OL]. 中华腔镜泌尿外科杂志(电子版), 2024, 18(01): 74-81.
[10] 赛甫丁·艾比布拉, 买买提·依斯热依力, 李义亮, 王永康, 王志, 克力木·阿不都热依木. 不同材质补片修补对腹壁疝大鼠腹横筋膜组织转化生长因子-β1及Collagen合成代谢的作用[J/OL]. 中华疝和腹壁外科杂志(电子版), 2024, 18(02): 161-167.
[11] 刘锦程, 王斌, 张雯, 张明周, 刘禹, 叶东樊, 黄赞胜, 邱凌霄, 卿斌, 王创业, 王南博, 王苹, 郭宇航, 周培花, 程秋霞, 徐智. 肺泡灌洗液RASSF1A及SHOX2甲基化联合径向超声特征对肺结节性质鉴别诊断的意义[J/OL]. 中华肺部疾病杂志(电子版), 2024, 17(04): 505-511.
[12] 李文哲, 潘鹏飞, 崔建, 于湘友. 体外生命支持技术临床应用的伦理学问题[J/OL]. 中华重症医学电子杂志, 2024, 10(02): 97-101.
[13] 萨仁高娃, 张英霞, 邓伟, 闫诺, 樊宁. 超声引导下鼠肝消融术后组织病理特征的变化规律及影响[J/OL]. 中华消化病与影像杂志(电子版), 2023, 13(06): 394-398.
[14] 郭子宾, 柯学锋, 余琳潇, 张伟艺, 张军, 汪娟. 2015年至2019年盐田区院外心脏骤停患者救治成功的影响因素分析和应对策略[J/OL]. 中华卫生应急电子杂志, 2024, 10(04): 199-202.
[15] 刘晴雯, 韩勇, 陈丽丹, 邓哲. 早期机械通气对成人院内心脏骤停病死率的影响：一项回顾性队列研究[J/OL]. 中华卫生应急电子杂志, 2024, 10(04): 203-206.
阅读次数
全文


摘要

版权所有 中华医学会 中华医学电子音像出版社有限责任公司  京ICP备14006079号-1  网络出版服务许可证：(署)网出证(京)字第075号

AI
小
编
AI小编
你好！我是《中华医学电子期刊资源库》AI小编，有什么可以帮您的吗？