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中华危重症医学杂志(电子版) ›› 2019, Vol. 12 ›› Issue (04) : 240 -244. doi: 10.3877/cma.j.issn.1674-6880.2019.04.005

所属专题: 文献

论著

结核分枝杆菌感染对非小细胞肺癌患者调节性免疫细胞的负向调节作用
宁洪叶1, 蒋贤高1,(), 施伎蝉1, 何贵清1, 吴正兴1   
  1. 1. 325000 浙江温州,温州市中心医院感染科
  • 收稿日期:2019-01-11 出版日期:2019-08-01
  • 通信作者: 蒋贤高

Negative regulation of Mycobacterium tuberculosis infection on regulatory immune cells in patients with non-small cell lung cancer

Hongye Ning1, Xiangao Jiang1,(), Jichan Shi1, Guiqing He1, Zhengxing Wu1   

  1. 1. Department of Infectious Diseases, Wenzhou Central Hospital, Wenzhou 325000, China
  • Received:2019-01-11 Published:2019-08-01
  • Corresponding author: Xiangao Jiang
  • About author:
    Corresponding author: Jiang Xiangao, Email:
引用本文:

宁洪叶, 蒋贤高, 施伎蝉, 何贵清, 吴正兴. 结核分枝杆菌感染对非小细胞肺癌患者调节性免疫细胞的负向调节作用[J/OL]. 中华危重症医学杂志(电子版), 2019, 12(04): 240-244.

Hongye Ning, Xiangao Jiang, Jichan Shi, Guiqing He, Zhengxing Wu. Negative regulation of Mycobacterium tuberculosis infection on regulatory immune cells in patients with non-small cell lung cancer[J/OL]. Chinese Journal of Critical Care Medicine(Electronic Edition), 2019, 12(04): 240-244.

目的

探讨结核分枝杆菌感染对非小细胞肺癌患者调节性免疫细胞的负向调节作用。

方法

选择2014年3月到2018年3月温州市中心医院就诊的非小细胞肺癌合并结核分枝杆菌感染患者为感染组(10例),单纯非小细胞肺癌患者为肿瘤组(10例),同期选择10例健康体检者为对照组。采用免疫组织化学法检测Ki-67增殖水平;采用流式细胞术检测CD4+CD25+Foxp3+调节性T(Treg)细胞和CD19+IL-10+调节性B(Breg)细胞的比例。

结果

肿瘤组Ki-67的比例为(35 ± 4)%,感染组为(66 ± 6)%,感染组Ki-67的比例显著高于肿瘤组(t = 4.068,P < 0.001)。三组肺癌合并结核患者外周血CD4+CD25+Foxp3+Treg细胞、CD19+IL-10+Breg细胞比例的比较,差异均有统计学意义(F = 69.400,P < 0.001;F = 16.090,P < 0.001)。且与肿瘤组CD4+CD25+Foxp3+Treg细胞比例[(0.41 ± 0.03)%、(0.21 ± 0.03)%、(1.03 ± 0.08)%]、CD19+IL-10+Breg细胞比例[(0.429 ± 0.021)%、(0.268 ± 0.013)%、(0.783 ± 0.111)%]比较,对照组均显著降低,而感染组均显著升高(P均< 0.05)。

结论

非小细胞肺癌合并结核分枝杆菌感染患者外周血中Treg细胞和Breg细胞比例显著升高。

Objective

To investigate the effect of Mycobacterium tuberculosis infection on regulatory immune cells in patients with non-small cell lung cancer (NSCLC).

Methods

The NSCLC patients in Wenzhou Central Hospital between March 2014 and March 2018 were selected as the infection group (n = 10) and cancer group (n = 10) according to whether suffering from Mycobacterium tuberculosis infection. Ten healthy subjects were enrolled as the control group at the same time. The proliferation level of Ki-67 was detected by immunohistochemistry. The flow cytometry was used to detect CD4+CD25+Foxp3+ regulatory T (Treg) cells and CD19+IL-10+ regulatory B (Breg) cells.

Results

The proportion of Ki-67 was (35 ± 4)% in the cancer group and (66 ± 6)% in the infection group; it was much higher in the infection group than in the cancer group (t = 4.068, P < 0.001). The proportion of CD4+CD25+Foxp3+Treg cells and CD19+IL-10+Breg cells among three groups all showed significant differences (F = 69.400, P < 0.001; F = 16.090, P < 0.001). Compared with the cancer group, the proportion of CD4+CD25+Foxp3+Treg cells [(0.41 ± 0.03)%, (0.21 ± 0.03)%, (1.03 ± 0.08)%] and CD19+IL-10+Breg cells[(0.429 ± 0.021)%, (0.268 ± 0.013)%, (0.783 ± 0.111)%] decreased markedly in the control group, and increased obviously in the infection group (all P < 0.05).

Conclusion

The proportion of Treg and Breg cells in peripheral blood of NSCLC patients with tuberculosis infection is significantly higher.

图1 非小细胞肺癌合并结核患者结核感染部位HE染色和抗酸染色结果
图2 肿瘤组和感染组患者癌细胞病理图
图3 各组患者Treg细胞比例的比较(n = 10)
图4 各组患者外周血Breg细胞比例的比较(n = 10)
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